| Products/Services Used | Details | Operation |
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| Gene Synthesis | Recombinant full-length Pvs48/45 (PlasmoDB accession number PVX_083235) was prepared as described (19). Briefly, the codon-harmonized sequence of Pvs48/45 (GenScript) was cloned between NdeI and NotI restriction sites of the expression vector pET(K-) modified to allow expression with an in-frame C-terminal 6× histidine tag. | Get A Quote |
Gamete surface protein P48/45 has been shown to be important for male gamete fertility and a strong candidate for the development of a malaria transmission-blocking vaccine (TBV). However, TBV development for Plasmodium vivax homolog Pvs48/45 has been slow because of a number of challenges: availability of conformationally suitable recombinant protein; the lack of an in vivo challenge model; and the inability to produce P. vivax gametocytes in culture to test transmission-blocking activity of antibodies. To support ongoing efforts to develop Pvs48/45 as a potential vaccine candidate, we initiated efforts to develop much needed reagents to move the field forward. We generated monoclonal antibodies (mAbs) directe... More
